Introduction to Light Microscopy：Tips and Tricks for Beginners

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Introduction to Light Microscopy：Tips and Tricks for Beginners

Microscopes are one of the most utilised and most ignored machines in the
lab. Being easy and reliable to use, many researchers and scientists don’t need
to put too much thought into what they are doing or the machine they are
using. That is, of course, until something goes wrong.
The goal of this book is to give microscope users a solid grounding in the
technology they are using. With a strong knowledge of the basics, the user will
be confident in approaching higher end systems and will be an invaluable
resource in any lab.
Over the next few chapters we shall break the microscope down into its
constituent parts, discuss how the part is assembled, how it works, and how
to use it best. To cover as many bases as possible I have included traditional
upright microscopes, geology microscopes, and inverted microscopes. I have
included guides on sample preparation for brightfield microscopy and more
general chapters such as consumables and what to consider when buying a
microscope. As much as possible I have used clear and simple language. Where
technical terms are required I have provided straightforward explanations.
The troubleshooting chapter will cover a few extra notes and methods to assist
in smooth imaging, but with a good introductory knowledge to microscopes,
the user will be capable of troubleshooting many issues themselves.
One of the best things about microscopes is that they are accessible to
everyone. No matter what the budget or requirement, there is a microscope
that fits. Everyone has equal access to this microworld, and my goal for this
book is to give every microscope user a clear field of view. Whether you are an
undergrad in university, a teacher trying to introduce young students to science,
or an armchair adventurer, I promise you this—once you take your first
look down the microscope, there will be no looking back.

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